MU Next Gen Sequencing Service

Genomics Core Services:
MU Next Gen Sequencing Service
Sanger DNA Sequencing Service
Shared Instrumentation
Bioinformatics Core Facility
CrossLab Instructions

Marshall University Next Generation Sequencing Service

Introduction

The Marshall University Genomics Core Facility provides Next Generation Sequencing (NGS) services to researchers throughout the state. The Genomics Core is equipped with an Illumina NextSeq 2000 sequencer with the capability to perform many high throughput sequencing applications including RNA-Seq (bulk and single cell), whole genome sequencing, whole exome sequencing, and ChIP-Seq. The NextSeq2000 can generate 1 billion single reads/run or 2 billion paired reads/run. In a 2 x100 paired end run, ~300 Gigabases can be generated. Many run configurations are possible

Investigators must meet with Core Personnel prior to any experimentation or sample preparation. At the initial meeting, Genomics Core staff and the investigator will define experimental design and objectives, technical issues (e.g. DNA/RNA quality and quantity, and library construction), probable sequence yields and outcomes, and overall cost.

Investigators must provide input DNA or RNA which meets minimum quality and quantity requirements. The Genomics Core will prepare standard Illumina or Illumina-compatible sequencing libraries based on established protocols. The Core will sequence libraries according to the experimental design and perform data analyses as requested by the investigator (e.g. variant calling and transcriptome analysis).

If you are interested in discussing a NGS project, please contact Don Primerano or Jim Denvir. To place a request, please complete a Genomics Core Analysis Request Form by logging into the MU Genomics and Bioinformatics Core interface at Agilent CrossLab (iLab Operations Software) and clicking on “Request Services”. Once you request has been placed, the Core Director will provide a quote for the requested services. You can also place orders for Sanger Sequencing and Bioanalyzer DNA/RNA analysis at the same site.

The Genomics Core also provides analysis of DNA and RNA samples on Agilent 2100 Bioanalyzers.

Contact Information: